P31946: 14-3-3 protein beta/alpha (Human)

Protein names
  • 14-3-3 protein beta/alpha
  • Protein 1054
  • Protein kinase C inhibitor protein 1 Short=KCIP-1
  • 1433B
Gene names YWHAB
Organism Homo sapiens
Protease Family (Not Available)
Protease ID (Not Available)
Chromosome 20

Isoforms

  • Isoform Short of 14-3-3 protein beta/alpha (P31946-2): sequence view | extended view

Sequence

        10         20         30         40         50         60 
MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS 
        70         80         90        100        110        120 
WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL ELLDKYLIPN ATQPESKVFY 
       130        140        150        160        170        180 
LKMKGDYFRY LSEVASGDNK QTTVSNSQQA YQEAFEISKK EMQPTHPIRL GLALNFSVFY 
       190        200        210        220        230        240 
YEILNSPEKA CSLAKTAFDE AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD 
   
AGEGEN

Annotation ?

Network neighborhood
[show protein-protein interactions]
 
100
200
Chains:
 
 
 
N-termini:

N
N
N
N
NN
N
N
N
N
NNN
 
C-termini:
C
CCC
C
 
Cleavage sites:
 
   
 
Features:
   
 
Sequence variations:
  
 
Modifications:
        
 
(MKAVT(32)|(33)EQGHE)
32,246

Cathepsin S (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance
inferred from experiment MEROPS Schilling O Biniossek ML et al.:Proteomic identification of pro... (C01.060) unknown none

Cathepsin L1 (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance
inferred from experiment MEROPS Schilling O Biniossek ML et al.:Proteomic identification of pro... (C01.060) unknown none

Targeted features: ?

32,246,246,1 CHAIN - 14-3-3 protein beta/alpha. (1|246)
32,246,246,2 CHAIN - 14-3-3 protein beta/alpha, N-terminally processed. (2|246)
 
(KAVTE(33)|(34)QGHEL)
33,246

Cathepsin S (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance
inferred from experiment MEROPS Schilling O Biniossek ML et al.:Proteomic identification of pro... (C01.060) unknown none

Targeted features: ?

33,246,246,1 CHAIN - 14-3-3 protein beta/alpha. (1|246)
33,246,246,2 CHAIN - 14-3-3 protein beta/alpha, N-terminally processed. (2|246)

Affected feature boundaries: ?

33,246,33,21 HELIX - (21|33)
 
(QTTVS(145)|(146)NSQQA)
145,246

Cathepsin L1 (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance
inferred from experiment MEROPS Schilling O Biniossek ML et al.:Proteomic identification of pro... (C01.060) unknown none

Targeted features: ?

145,246,246,1 CHAIN - 14-3-3 protein beta/alpha. (1|246)
145,246,246,2 CHAIN - 14-3-3 protein beta/alpha, N-terminally processed. (2|246)
145,246,161,139 HELIX - (139|161)
 
acetylation-1MTMDKS...
1,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
inferred from electronic annotation UniProtKB inferred from uniprot unknown unknown 0.0 (unknown)
Gevaert K. Van Damme P et al.: Complementary positional proteomics for screening substrates... direct yes 0.0 (unknown)
Gevaert K. Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates... direct yes 0.0 (unknown)
Overall Laboratory Lange_Huesgen_et_al-RBC direct yes 0.88 (PeptideProphet probability)

Affected feature boundaries: ?

1,246,2,1 VAR_SEQ - Missing (in isoform Short). (1|2)
1,246,1,1 INIT_MET - Removed; alternate. (1|1)
1,246,1,1 MOD_RES - N-acetylmethionine; in 14-3-3 protein beta/alpha; alternate. (1|1)
1,246,246,1 CHAIN - 14-3-3 protein beta/alpha. (1|246)
 
unknown-1MTMDKS...
1,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
Wells Jurkat_untreated direct yes 0.02 (ProteinProspector)
Wells apoptotic_Jurkat_staurotrail direct yes 0.02 (ProteinProspector)

Affected feature boundaries: ?

1,246,2,1 VAR_SEQ - Missing (in isoform Short). (1|2)
1,246,1,1 INIT_MET - Removed; alternate. (1|1)
1,246,1,1 MOD_RES - N-acetylmethionine; in 14-3-3 protein beta/alpha; alternate. (1|1)
1,246,246,1 CHAIN - 14-3-3 protein beta/alpha. (1|246)
 
acetylation-2TMDKSE...
2,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
inferred from electronic annotation UniProtKB inferred from uniprot unknown unknown 0.0 (unknown)
Gevaert K. Van Damme P et al.: Complementary positional proteomics for screening substrates... direct yes 0.0 (unknown)
Gevaert K. Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates... direct yes 0.0 (unknown)
Overall Laboratory Lange_Huesgen_et_al-RBC direct yes 0.88 (PeptideProphet probability)

Affected feature boundaries: ?

2,246,2,2 MOD_RES - N-acetylthreonine; in 14-3-3 protein beta/alpha, N-terminally processed. (2|2)
2,246,246,2 CHAIN - 14-3-3 protein beta/alpha, N-terminally processed. (2|246)
 
unknown-3MDKSEL...
3,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
inferred from isoform by sequence similarity TopFIND inferred from TNt65718 indirect unknown (unknown)
 
acetylation-3MDKSEL...
3,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
Overall Laboratory Lange_Huesgen_et_al-RBC direct yes 0.88 (PeptideProphet probability)
inferred from isoform by sequence similarity TopFIND inferred from TNt182635 indirect unknown (unknown)
 
acetylation-30AVTEQG...
30,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
Overall Laboratory Lange_Huesgen_et_al-RBC direct yes 0.88 (PeptideProphet probability)
inferred from isoform by sequence similarity TopFIND inferred from TNt182637 indirect unknown (unknown)
 
unknown-30AVTEQG...
30,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
Wells apoptotic_Jurkat_bortezomib direct yes 0.02 (ProteinProspector)
Wells apoptotic_Jurkat_staurotrail direct yes 0.02 (ProteinProspector)
Wells apoptotic_MM1s_bort direct yes 0.02 (ProteinProspector)
Wells apoptotic_MM1SDBJurkat_Mix direct yes 0.02 (ProteinProspector)
Wells apoptosis_U266_bortezomib_induced direct yes 0.02 (ProteinProspector)
 
unknown-30AVTEQG...
30,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
inferred from isoform by sequence similarity TopFIND inferred from TNt166826 indirect unknown (unknown)
 
unknown-33EQGHEL...
33,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
inferred from cleavage TopFIND Inferred from cleavage TC7440 indirect unknown (unknown)
inferred from cleavage TopFIND Inferred from cleavage TC5797 indirect unknown (unknown)
inferred from isoform by sequence similarity TopFIND inferred from TNt137585 indirect unknown (unknown)
 
pyrrolidone carboxylic acid-33EQGHEL...
33,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
Overall Laboratory Lange_Huesgen_et_al-RBC direct yes 0.88 (PeptideProphet probability)
inferred from isoform by sequence similarity TopFIND inferred from TNt182639 indirect unknown (unknown)
 
unknown-34QGHELS...
34,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
inferred from cleavage TopFIND Inferred from cleavage TC7442 indirect unknown (unknown)
inferred from isoform by sequence similarity TopFIND inferred from TNt137670 indirect unknown (unknown)
 
acetylation-44NLLSVA...
44,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
Overall Laboratory Lange_Huesgen_et_al-RBC direct yes 0.88 (PeptideProphet probability)
inferred from isoform by sequence similarity TopFIND inferred from TNt182641 indirect unknown (unknown)
 
unknown-146NSQQAY...
146,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
inferred from cleavage TopFIND Inferred from cleavage TC5799 indirect unknown (unknown)
inferred from isoform by sequence similarity TopFIND inferred from TNt144496 indirect unknown (unknown)
 

Protein C-Termini [export]

...MKAVT32-unknown
32,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
inferred from cleavage TopFIND Inferred from cleavage TC7440 indirect unknown (unknown)
inferred from cleavage TopFIND Inferred from cleavage TC5797 indirect unknown (unknown)
inferred from isoform by sequence similarity TopFIND inferred from TCt120576 indirect unknown (unknown)
 
...KAVTE33-unknown
33,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
inferred from cleavage TopFIND Inferred from cleavage TC7442 indirect unknown (unknown)
inferred from isoform by sequence similarity TopFIND inferred from TCt120667 indirect unknown (unknown)

Affected feature boundaries: ?

33,246,33,21 HELIX - (21|33)
 
...QTTVS145-unknown
145,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
inferred from cleavage TopFIND Inferred from cleavage TC5799 indirect unknown (unknown)
inferred from isoform by sequence similarity TopFIND inferred from TCt127761 indirect unknown (unknown)
 
...GEGEN246-unknown
246,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
inferred from electronic annotation UniProtKB inferred from uniprot unknown unknown 0.0 (unknown)
inferred from isoform by sequence similarity TopFIND inferred from TCt61336 indirect unknown (unknown)
Gevaert K. Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates... direct yes 0.0 (unknown)

Affected feature boundaries: ?

246,246,246,1 CHAIN - 14-3-3 protein beta/alpha. (1|246)
246,246,246,2 CHAIN - 14-3-3 protein beta/alpha, N-terminally processed. (2|246)
 
...GEGEN246-unknown
246,246

Evidence: (more...)

Evidence ? Source (database) ? Source (laboratory) ? Name ? Directness of identification ? Phys Relevance Confidence
Gevaert K. Van Damme P et al.: PC3-cells, Complementary positional proteomics for screening substrates... direct yes 0.0 (unknown)

Affected feature boundaries: ?

246,246,246,1 CHAIN - 14-3-3 protein beta/alpha. (1|246)
246,246,246,2 CHAIN - 14-3-3 protein beta/alpha, N-terminally processed. (2|246)
 
Filter by evidence: ?

Directness ?

  • unknown
  • direct
  • indirect

Physiological relevance ?

  • none
  • unknown
  • yes

Evidencecode ?

Method ?

Perturbation ?

Confidence greater than?

  • unknown
  • PeptideProphet probability
  • ProteinProspector

Experimental system ?

Certainty of Protease assignment ?

  • unknown
  • IV proteolytic system present and active

Evidencecode ?

Tissue distribution ?

Specific evidence ?

Derived from database?

Laboratory ?